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the structural organization of actin and focal adhesions

Cells

HUVECs were isolated by collagenase (Roche Diagnostics) digestion.17 Cells were routinely grown in flasks coated with 0.2% gelatin in M199 medium containing 2 mM glutamine, 50 U/mL penicillin, 50 μg/mL streptomycin, 2.5 μg/mL amphotericin B, 7.5% NHS, and 7.5% FBS (regular medium). HUVECs from the second or third passage were used. The human colorectal carcinoma cells DLD-1 were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in RPMI supplemented with 10% FBS. Human aortic smooth muscle cells (hASMCs) and the corresponding growth medium (SmGM-2) were purchased from Clonetics (BioWhittaker, Emerainville, France). Normal human dermal fibroblasts (NHDFs) and fibroblast growth medium (FGM) were purchased from Promocell