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Talaromyces wortmannii. the fungal growth in large scale

Among the hits retrieved by the database, the presence of multicolanic acid, (+)-mitorubrin, (−)-mitorubrinol, (+)-mitorubrinol acetate, skyrin, rugulosin, and rugulovasine A and B, was confirmed in the chromatograms by the comparison with the authentic reference standards. The overview from the confirmed AntiBase hits produced by T. wortmannii in PDA medium is shown in Still including the main detected peaks, the 8-chlororugulovasines A and B were also found as likely records in the database. Since those compounds were not available in the standard collection, they were isolated from the scaled-up cultivation of the strain and confirmed on the basis of spectroscopic analysis, as will be further described. Apart from the identified compounds, two peaks eluted around 3.7 to 3.9 min in the BPC chromatogram did not provide potential candidates, either in AntiBase or in the further literature queries. These observations led to the hypothesis of compounds biosynthesized by T. wortmanniistill to be elucidated.

2.1. Known and New Halogenated Rugulovasines Produced by T. wortmannii

Besides the production of rugulovasine A and B by T. wortmannii, the dereplication process pointed towards the co-production of the halocongeners 8-chlororugulovasines A and B. This evidence was provided by the detection of the accurate masses m/z 303.0892 (−1.0 ppm) and 303.0896 (−0.3 ppm) where the elementary compositions were given by the same formula C16H16O2N2Cl. The occurrence of halogenated compounds in the fungal micro-extracts was suggested not only by the likely detection of the known monochlorinated alkaloids but also due to the isotope pattern displayed by the two unknown ions detected at 3.7 and 3.9 min Their accurate masses m/z 337.0506 (−0.2 ppm) and 337.0507 (−0.6 ppm) afforded the molecular formula C16H15O2N2Cl2 for both, which, notably, matched to two additional chlorine atoms when compared to rugulovasines chemical composition (C16H17O2N2). Indeed, in accordance with the theoretical isotopic pattern found for the C16H15O2N2Cl2, the observed ions fitted to dichlorinated species