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Homogeneous pattern of tubule lengths

All cell culture reagents were purchased from Life Technologies (Rockville, MD) unless otherwise specified. Normal human AB male serum (NHS) and fetal bovine serum (FBS) were obtained from Biowest (Cholet, France). Rabbit polyclonal anti–c-met IgG C-28 raised against a peptide mapping at the carboxy terminus of c-met, blocking peptide for IgG C-28, and rabbit polyclonal IgG H-190 raised against a recombinant protein mapping within the extracellular domain of c-met were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). A mouse monoclonal antibody against VE-cadherin, clone TEA 1/31, was purchased from Immunotech (Marseille, France). A fluorescein isothiocyanate (FITC)-conjugated antibody against rabbit IgG, a tetrarhodamine isothiocyanate (TRITC)–conjugated antibody against mouse IgG, a peroxidase-conjugated antibody against rabbit IgG, and a control rabbit IgG were all purchased from Sigma (St Louis, MO). A peroxidase-conjugated antibody against mouse IgG was purchased from Jackson Immunoresearch Laboratories (West Grove, PA). Recombinant human HGF, HGF R/Fc chimera (c-met extracellular domain fused to human IgG1 Fc), and platelet-derived growth factor (PDGF) BB were purchased fromR&D Systems (Minneapolis, MN). The BCA protein assay reagent was purchased from Pierce (Rockford, IL) and the cell proliferation reagent WST-1 was purchased from Roche Diagnostics (Mannheim, Germany). A mouse monoclonal antiactin antibody, nitrocellulose Hybond C Extra membrane, enhanced chemiluminescence (ECL) plus Western blotting detection reagent, and [methyl3H]thymidine (74 GBq/mmol) were all purchased from Amersham Biosciences (Buckinghamshire, United Kingdom).