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function of the influence of the fungus atom

The fungus T. wortmannii (CML 2704) was isolated from symptomless apple fruits (Malus domestica), following standard procedures for isolation of endophytic fungi [21]. Different growth media were tested for the incubation of the microorganism: PDA, CREA, MEA, CYA, DRYES, MEAox, WATM, YES and OA (for definitions see Section 3.5). The obtained micro-extracts had their metabolite screening carried out by UHPLC-DAD-QTOFMS (quadrupole time-of-flight mass detector) data analysis. A thorough investigation was applied from the observed accurate monoisotopic masses, fragment ions and adduct patterns, which assisted the finding of the elemental composition from the detected peaks in the base peak chromatograms (BPC). The found molecular formulas and accurate masses (±5 ppm) in the database AntiBase 2012 [22] as well as the use of a comprehensive microbial standard collection [9,10] were concomitantly applied along the data analysis process. Owing to the similar chemical profile exhibited in the chromatograms from the investigated micro-extracts, the PDA medium was chosen as the working growth medium and likewise for the results discussed hereafter.

Molecules 20 17627 g002 550

Figure 2. BPC chromatogram (100–1000 Da) from the micro-extract of T. wortmannii growth in PDA medium. Compounds identified by UV/Vis-HRMS based dereplication are indicated. (*) Isolated compounds. (**) compounds confirmed by comparison with reference standards. Peaks not highlighted have not been unambiguously identified. Data acquired at electrospray ionization in positive mode (ESI+), UHPLC-DAD-QTOFMS instrument.