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amphipod Crangonyx pseudogracilis

t his study examines the freshwater amphipod Crangonyx pseudogracilis as a potential new indicator species when used in the Multispecies Freshwater Biomonitor (MFB). The MFB is an online continuous biomonitor which uses impedance conversion to record behavioral responses of vertebrates and invertebrates. Four experiments were undertaken to establish: (1) if the electrical field generated by the MFB affected the organisms’ behavior, (2) if defined behaviors and their response to a gradient of ammonium chloride could be detected by the MFB, (3) if there was variation in the behavior of C. pseudogracilis over a diel cycle, and (4) if behavior changed significantly in response to a pulse of ammonium chloride. Results showed no significant effect of the MFB’s current on behavior of C. pseudogracilis. Four behaviors; swimming, walking, grooming and inactivity, were observed and identified in the MFB. In the MFB, each behavior changed significantly in response to an increasing gradient of ammonium chloride exposure. The MFB also detected increases in nocturnal activity by C. pseudogracilis. The MFB also detected a significant increase in activity after a pulse of ammonium chloride …